Fig. 2
From: Ovulatory signals alter granulosa cell behavior through YAP1 signaling
Effect of verteporfin and oocytes on monolayer granulosa cell survival. a. Optical density of granulosa cells cultured in medium containing low (0.5%) fetal bovine serum (control) or medium supplemented with oocytes (2 oocytes/μl) and/or verteporfin (200 nM) for 48 h. b. YAP1 localization in cumulus granulosa cells cultured alone for 24 h and stained for total YAP1 and DNA (DAPI). c. YAP1 localization in cumulus granulosa cells cultured with oocytes (4 oocytes/μl) for 24 h and stained for total YAP1 and DNA (DAPI). Values are mean ± SEM of background subtracted optical density readings. A,B,C Indicate significant differences by one-way ANOVA followed by Tukey’s post-hoc test, P < 0.01, N = 3